CBI chief Alok Verma, his deputy Rakesh Asthana and several other officers have been sent on leave in an extraordinary coda to a battle within the country's top investigating agency.
M Nageshwar Rao took over as interim director very early on Wednesday, hours after a midnight government order.
Nageshwar Rao, a 1986 batch officer, supersedes Rakesh Asthana and was joint director of the CBI or Central Bureau of Investigation.
Mr Rao will "look after duties and functions of director CBI and shall take over the duties and functions with immediate effect," said the order by an appointments committee led by the Prime Minister.
The offices of both Alok Verma and Rakesh Asthana have been sealed and all officers working with the CBI director have been sent on leave.
The rooms of all these officers on the 10th and 11th floor of the CBI's headquarters in Delhi have been sealed and are being searched, say sources.
The developments followed a recommendation by the country's top vigilance officer, Chief Vigilance Commissioner KV Chowdary that the CBI's warring top two be sent on "compulsory wait", stripped of all responsibilities.
Sources say Prime Minister Narendra Modi, who had summoned both Alok Verma and Rakesh Asthana to discuss the crisis, agreed with the recommendation.
The move caps days of turmoil that began with chief Alok Verma filing a case against his deputy, accusing him of taking Rs. 3 crore as bribe from a businessman being investigated.
Rakesh Asthana, denying the charges, told the government that it was his boss who had taken a bribe of Rs. 2 crore from the same businessman. He also sent a 50-page complaint against Alok Verma and his team.
The opposition has accused the government of trying to protect Rakesh Asthana - a Gujarat cadre officer dubbed the Prime Minister's "blue-eyed boy" by Congress president Rahul Gandhi - who, after being appointed the number two officer last year despite stiff opposition from Alok Verma, handled major investigations.
Alok Verma cannot be removed before his fixed two-year term as CBI director without a meeting of a group that includes the PM, Chief Justice and leader of opposition, said lawyer-activist Prashant Bhushan.
Rakesh Asthana yesterday took the CBI to court, asking for the charges against him to be cancelled and for protection from arrest.
The Delhi High Court did not cancel the case, but sent notice to the CBI director and said Rakesh Asthana would not be arrested at least until it heard the case next Monday.